The interaction between tazobactam and several chromosome- and plasmid-encoded (TEM, SHV, PSE types) class A and C β-lactamases was studied by spectrophotometry. Tazobactam behaved as a competitive inhibitor or inactivator able to restore in several cases the efficiency of piperacillin as a partner β-lactam. A detailed kinetic analysis permitted measurement of the acylation efficiency for some cephalosporinases and broad-spectrum β-lactamases; the presence of a turn-over of acyl-enzyme complex was also evaluated.

A kinetic study on the interaction between tazobactam (a penicillanic acid sulphone derivative) and active-site serine β-lactamases

PERILLI, MARIAGRAZIA;FRANCESCHINI, Nicola;SEGATORE, Bernardetta;AMICOSANTE, Gianfranco
1999-01-01

Abstract

The interaction between tazobactam and several chromosome- and plasmid-encoded (TEM, SHV, PSE types) class A and C β-lactamases was studied by spectrophotometry. Tazobactam behaved as a competitive inhibitor or inactivator able to restore in several cases the efficiency of piperacillin as a partner β-lactam. A detailed kinetic analysis permitted measurement of the acylation efficiency for some cephalosporinases and broad-spectrum β-lactamases; the presence of a turn-over of acyl-enzyme complex was also evaluated.
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/11697/6747
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