An efficient over-expression system was developed for CTX-M-3 extended-spectrum-β-lactamase. The recombinant enzyme was purified from 1 l of culture to yield 22 mg of pure enzyme. The N-terminal amino acid sequence was determined to be NH 2-QTADVQ... Determination of kinetic parameters with the purified CTX-M-3 revealed efficient hydrolysis of penicillins and cephalosporins, while ceftazidime and aztreonam were very poor substrates. Clavulanic acid, sulbactam and especially tazobactam inhibited the CTX-M-3 enzyme. © 2004 Federation of European Microbiological Societies. Published by Elsevier B.V. All rights reserved.
|Titolo:||Overexpression system and biochemical profile of CTX-M-3 extended-spectrum β-lactamase expressed in Escherichia coli|
|Autori interni:||PERILLI, MARIAGRAZIA|
|Data di pubblicazione:||2004|
|Rivista:||FEMS MICROBIOLOGY LETTERS|
|Appare nelle tipologie:||1.1 Articolo in rivista|