The Aeromonas hydrophila CphA metallo-β-lactamase was overexpressed in a soluble secreted form in Escherichia coli using a T7 RNA polymerase-based expression system, and a simple protocol based on a single cation-exchange chromatographic step was developed, which is suitable for rapid purification of the overexpressed enzyme from E. coli lysates. A yield of up to 30 μg of purified enzyme per milliliter of culture was obtained. The purified enzyme preparation showed properties identical to those previously reported in the literature.
|Titolo:||Overproduction and purification of the Aeromonas hydrophila CphA metallo-β-lactamase expressed in Escherichia coli|
|Autori interni:||PERILLI, MARIAGRAZIA|
|Data di pubblicazione:||1996|
|Rivista:||MICROBIAL DRUG RESISTANCE|
|Appare nelle tipologie:||1.1 Articolo in rivista|