Two chromosome-encoded beta-lactamases have been purified from Citrobacter diversus ULA-27. They exhibited slightly different isoelectric points (6.8 and 6.2) and very similar Mr values (congruent to 29,000). Their specificity spectrum was rather wide, since they hydrolysed some cephalosporins with kcat: values similar to those observed with the best penicillin substrates. Cloxacillin, methicillin and imipenem were hydrolysed very slowly. Hydrolysis of azthreonam could not be detected.
|Titolo:||Citrobacter diversus ULA-27 beta-lactamases. Improved purification and general properties|
|Autori interni:||AMICOSANTE, Gianfranco|
|Data di pubblicazione:||1988|
|Appare nelle tipologie:||1.1 Articolo in rivista|