A survey of the truffle Tuber melanosporum genome has shown the presence of 67 programmed cell death (PCD) related genes. The 67 genes are all expressed during fruit body (FB) development of T. melanosporum; their expression has been detected by DNA microarrays and qPCR. A set of fourteen PCD related genes have been chosen, those with the highest identities to the homologs of other species, for a deeper investigation. That PCD occurs during T. melanosporum development has been demonstrated by the TUNEL reaction and transmission electron microscopy. The findings of this work, in addition to the discovery of PCD related genes in the T. melanosporum genome and their expression during the differentiation and development of the fruit body (FB) would suggest that one of the PCD subroutines, may be autophagy, is involved in the FB ripening, i.e. sporogenesis.

The genomic tool-kit of the truffle Tuber melanosporum programmed cell death

Osvaldo Zarivi;Patrizia Cesare;Anna Poma;Sabrina Colafarina;Antonella Bonfigli;Pierpaolo Aimola;Anna Ragnelli;Giovanni Pacioni;Michele Miranda
2018

Abstract

A survey of the truffle Tuber melanosporum genome has shown the presence of 67 programmed cell death (PCD) related genes. The 67 genes are all expressed during fruit body (FB) development of T. melanosporum; their expression has been detected by DNA microarrays and qPCR. A set of fourteen PCD related genes have been chosen, those with the highest identities to the homologs of other species, for a deeper investigation. That PCD occurs during T. melanosporum development has been demonstrated by the TUNEL reaction and transmission electron microscopy. The findings of this work, in addition to the discovery of PCD related genes in the T. melanosporum genome and their expression during the differentiation and development of the fruit body (FB) would suggest that one of the PCD subroutines, may be autophagy, is involved in the FB ripening, i.e. sporogenesis.
File in questo prodotto:
File Dimensione Formato  
Zarivi_et_al-2018-Cell_Death_Discovery.pdf

accesso aperto

Tipologia: Documento in Versione Editoriale
Licenza: Dominio pubblico
Dimensione 1.85 MB
Formato Adobe PDF
1.85 MB Adobe PDF Visualizza/Apri

I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.

Utilizza questo identificativo per citare o creare un link a questo documento: http://hdl.handle.net/11697/119315
Citazioni
  • ???jsp.display-item.citation.pmc??? ND
  • Scopus 1
  • ???jsp.display-item.citation.isi??? 1
social impact