In vitro immune effects of Pt compounds of occupational and/or environmental importance, or those used in cancer treatment were studied. Spontaneous and PHA-stimulated proliferation of peripheral blood mononuclear cells (PBMC) and in vitro release of TNF-α, IFN-γ, and IL-5 were assessed in presence of high and very low concentrations of Pt salts: 10-4 and 10-7 M (NH4)2[PtCl6], (NH4)2[PtCl4], PtCl4, PtCl2, Na2PtI6, and cis-diaminedichloroPt (CisPt). Spontaneous and PHA-stimulated PBMC proliferation were both inhibited by 10-4 M (NH4)2[PtCl6] and (NH4)2[PtCl4], while only PHA-stimulated proliferation was inhibited by 10-4 M CisPt, without significant effects of the other Pt salts. TNF-α release from PBMC was reduced by 10-4 M (NH4)2[PtCl6] and INF-γ release was reduced by 10-4 and 10-7 M hexa- and tetrachloroplatinate and 10-4 M Na2PtI6, but not by other Pt salts. IL-5 release (related to the Th2 immune response) was inhibited by 10-4 M (NH4)2[PtCl6], (NH4)2[PtCl4] and Na2PtI6, but it was enhanced by both 10-4 and 10-7 M PtCl4. PtCl2 did not influence the immune effects. The study shows Pt salts have immune effects and their potency is ranked in the following order: (NH4)2[PtCl6] > (NH4)2[PtCl4] > Na2PtI6 and CisPt > PtCl4 > PtCl2. These results indicate that certain Pt salts affect lymphocyte proliferation and cytokine release. The intracellular mechanisms responsible for such effects have not been identified.

In vitro effects of platinum compounds on lymphocyte proliferation and cytokine release

VOLPE, ANNA RITA;CARMIGNANI, Marco;
2004

Abstract

In vitro immune effects of Pt compounds of occupational and/or environmental importance, or those used in cancer treatment were studied. Spontaneous and PHA-stimulated proliferation of peripheral blood mononuclear cells (PBMC) and in vitro release of TNF-α, IFN-γ, and IL-5 were assessed in presence of high and very low concentrations of Pt salts: 10-4 and 10-7 M (NH4)2[PtCl6], (NH4)2[PtCl4], PtCl4, PtCl2, Na2PtI6, and cis-diaminedichloroPt (CisPt). Spontaneous and PHA-stimulated PBMC proliferation were both inhibited by 10-4 M (NH4)2[PtCl6] and (NH4)2[PtCl4], while only PHA-stimulated proliferation was inhibited by 10-4 M CisPt, without significant effects of the other Pt salts. TNF-α release from PBMC was reduced by 10-4 M (NH4)2[PtCl6] and INF-γ release was reduced by 10-4 and 10-7 M hexa- and tetrachloroplatinate and 10-4 M Na2PtI6, but not by other Pt salts. IL-5 release (related to the Th2 immune response) was inhibited by 10-4 M (NH4)2[PtCl6], (NH4)2[PtCl4] and Na2PtI6, but it was enhanced by both 10-4 and 10-7 M PtCl4. PtCl2 did not influence the immune effects. The study shows Pt salts have immune effects and their potency is ranked in the following order: (NH4)2[PtCl6] > (NH4)2[PtCl4] > Na2PtI6 and CisPt > PtCl4 > PtCl2. These results indicate that certain Pt salts affect lymphocyte proliferation and cytokine release. The intracellular mechanisms responsible for such effects have not been identified.
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/11697/11957
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