Several studies have provided information on environmental nanoplastic particles/debris, but the in vitro cyto-genotoxicity is still insuciently characterized. The aim of this study is to analyze the eects of polystyrene nanoparticles (PNPs) in the Hs27 cell line. The viability of Hs27 cells was determined following exposure at dierent time windows and PNP concentrations. The genotoxic eects of the PNPs were evaluated by the cytokinesis-block micronucleus (CBMN) assay after exposure to PNPs. We performed ROS analysis on HS27 cells to detect reactive oxygen species at dierent times and treatments in the presence of PNPs alone and PNPs added to the Crocus sativus L. extract. The dierent parameters of the CBMN test showed DNA damage, resulting in the increased formation of micronuclei and nuclear buds. We noted a greater increase in ROS production in the short treatment times, in contrast, PNPs added to Crocus sativus showed the ability to extract, thus reducing ROS production. Finally, the SEM-EDX analysis showed a three-dimensional structure of the PNPs with an elemental composition given by C and O. This work defines PNP toxicity resulting in DNA damage and underlines the emerging problem of polystyrene nanoparticles, which extends transversely from the environment to humans; further studies are needed to clarify the internalization process.

In Vitro Genotoxicity of Polystyrene Nanoparticles on the Human Fibroblast Hs27 Cell Line

Anna Poma
;
Giulia Vecchiotti;Sabrina Colafarina;Osvaldo Zarivi;Lorenzo Arrizza;Giuseppe Chichiriccò;Piero Di Carlo
2019

Abstract

Several studies have provided information on environmental nanoplastic particles/debris, but the in vitro cyto-genotoxicity is still insuciently characterized. The aim of this study is to analyze the eects of polystyrene nanoparticles (PNPs) in the Hs27 cell line. The viability of Hs27 cells was determined following exposure at dierent time windows and PNP concentrations. The genotoxic eects of the PNPs were evaluated by the cytokinesis-block micronucleus (CBMN) assay after exposure to PNPs. We performed ROS analysis on HS27 cells to detect reactive oxygen species at dierent times and treatments in the presence of PNPs alone and PNPs added to the Crocus sativus L. extract. The dierent parameters of the CBMN test showed DNA damage, resulting in the increased formation of micronuclei and nuclear buds. We noted a greater increase in ROS production in the short treatment times, in contrast, PNPs added to Crocus sativus showed the ability to extract, thus reducing ROS production. Finally, the SEM-EDX analysis showed a three-dimensional structure of the PNPs with an elemental composition given by C and O. This work defines PNP toxicity resulting in DNA damage and underlines the emerging problem of polystyrene nanoparticles, which extends transversely from the environment to humans; further studies are needed to clarify the internalization process.
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Utilizza questo identificativo per citare o creare un link a questo documento: http://hdl.handle.net/11697/138026
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