Background: Pharmacological treatment approaches for eating disorders, such as binge eating disorder and bulimia nervosa, are currently limited. Methods and aims: Using a well-characterized animal model of binge eating, we investigated the epigenetic regulation of the A(2A) Adenosine Receptor (A(2A)AR) and dopaminergic D2 receptor (D2R) genes. Results: Gene expression analysis revealed a selective increase of both receptor mRNAs in the amygdaloid complex of stressed and restricted rats, which exhibited binge-like eating, when compared to non-stressed and non-restricted rats. Consistently, pyrosequencing analysis revealed a significant reduction of the percentage of DNA methylation but only at the A(2A)AR promoter region in rats showing binge-like behaviour compared to the control animals. Focusing thus on A(2A)AR agonist (VT 7) administration (which inhibited the episode of binge systemically at 0.1 mg/kg or intra-central amygdala (CeA) injection at 900 ng/side) induced a significant increase of A(2A)AR mRNA levels in restricted and stressed rats when compared to the control group. In addition, we observed a significant decrease in A(2A)AR mRNA levels in rats treated with the A(2A)AR antagonist (ANR 94) at 1 mg/kg. Consistent changes in the DNA methylation status of the A(2A)AR promoter were found in restricted and stressed rats after administration of VT 7 or ANR 94. Conclusion: We confirm the role of A(2A)AR in binge eating behaviours, and we underline the importance of epigenetic regulation of the A(2A)AR gene, possibly due to a compensatory mechanism to counteract the effect of binge eating. We suggest that A(2A)AR activation, inducing receptor gene up-regulation, could be relevant to reduction of food consumption.

Regulation of adenosine A(2A) receptor gene expression in a model of binge eating in the amygdaloid complex of female rats

Maccarrone M;
2019

Abstract

Background: Pharmacological treatment approaches for eating disorders, such as binge eating disorder and bulimia nervosa, are currently limited. Methods and aims: Using a well-characterized animal model of binge eating, we investigated the epigenetic regulation of the A(2A) Adenosine Receptor (A(2A)AR) and dopaminergic D2 receptor (D2R) genes. Results: Gene expression analysis revealed a selective increase of both receptor mRNAs in the amygdaloid complex of stressed and restricted rats, which exhibited binge-like eating, when compared to non-stressed and non-restricted rats. Consistently, pyrosequencing analysis revealed a significant reduction of the percentage of DNA methylation but only at the A(2A)AR promoter region in rats showing binge-like behaviour compared to the control animals. Focusing thus on A(2A)AR agonist (VT 7) administration (which inhibited the episode of binge systemically at 0.1 mg/kg or intra-central amygdala (CeA) injection at 900 ng/side) induced a significant increase of A(2A)AR mRNA levels in restricted and stressed rats when compared to the control group. In addition, we observed a significant decrease in A(2A)AR mRNA levels in rats treated with the A(2A)AR antagonist (ANR 94) at 1 mg/kg. Consistent changes in the DNA methylation status of the A(2A)AR promoter were found in restricted and stressed rats after administration of VT 7 or ANR 94. Conclusion: We confirm the role of A(2A)AR in binge eating behaviours, and we underline the importance of epigenetic regulation of the A(2A)AR gene, possibly due to a compensatory mechanism to counteract the effect of binge eating. We suggest that A(2A)AR activation, inducing receptor gene up-regulation, could be relevant to reduction of food consumption.
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Utilizza questo identificativo per citare o creare un link a questo documento: http://hdl.handle.net/11697/155792
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