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EnglishWe found, by circular dichroism and Raman spectroscopy measurements. that the secondary structure of the native ovalbumin and of its heat-stable form, called S-ovalbumin, is a probe of the structural differences between the two proteins. Small angle X-ray scattering and circular dichroism measurements performed on the two proteins under denaturing conditions, with different concentrations of guanidine hydrochloride, show the changes of the tertiary and secondary structure and a different pathway in the unfolding process. These experimental data confirm that the conversion of native ovalbumin into S-ovalbumin is irreversible and reveal that the response of the two proteins to the same chemical environment is different.
| Titolo: | Structural differences of ovalbumin and S-ovalbumin revealed by denaturing conditions |
| Autori: | |
| Data di pubblicazione: | 1997 |
| Rivista: | |
| Abstract: | We found, by circular dichroism and Raman spectroscopy measurements. that the secondary structure of the native ovalbumin and of its heat-stable form, called S-ovalbumin, is a probe of the structural differences between the two proteins. Small angle X-ray scattering and circular dichroism measurements performed on the two proteins under denaturing conditions, with different concentrations of guanidine hydrochloride, show the changes of the tertiary and secondary structure and a different pathway in the unfolding process. These experimental data confirm that the conversion of native ovalbumin into S-ovalbumin is irreversible and reveal that the response of the two proteins to the same chemical environment is different. |
| Handle: | http://hdl.handle.net/11697/16418 |
| Appare nelle tipologie: | 1.1 Articolo in rivista |
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