The use of a high energy laser source for Soft X-Ray Contact Microscopy (SXCM) is discussed. Different targets have been used and their emission spectra recorded and compared inside and outside the Water Window spectral region. Up to twelve sample holders per shot have been exposed, thanks to the large X-ray flux and the geometry of the interaction chamber. Images of biological samples have been obtained, including Chlamydomonas, Phytomonas, Saccaromyces Cerevisiae yeast cells and pig sperm. A 50 nm resolution has been achieved and original biological information concerning the inner structure of the cells has been obtained.
Contact X-ray Microscopy using the Asterix laser source
PALLADINO, Libero
2000-01-01
Abstract
The use of a high energy laser source for Soft X-Ray Contact Microscopy (SXCM) is discussed. Different targets have been used and their emission spectra recorded and compared inside and outside the Water Window spectral region. Up to twelve sample holders per shot have been exposed, thanks to the large X-ray flux and the geometry of the interaction chamber. Images of biological samples have been obtained, including Chlamydomonas, Phytomonas, Saccaromyces Cerevisiae yeast cells and pig sperm. A 50 nm resolution has been achieved and original biological information concerning the inner structure of the cells has been obtained.File in questo prodotto:
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