This study concerns the biochemical and physicochemical behaviour of liposomes as enzymatic bioreactors. The aim is to study a quantitative treatment of transport/kinetic phenomena in these bioreactors. The experimental system studied is the enzyme ascorbate oxidase (AAO) entrapped into dipalmitoyl-phosphatidylcholine unilamellar vesicles. The vesicles were prepared by two different methods, namely by controlled detergent dialysis technique or by reverse phase technique. A kinetic model which considers the presence of AAO both on the surface and in the aqueous core of the liposomes has been developed.
Liposomes as bioreactors. Transport/kinetic phenomena
DI GIULIO, Antonio;
1990-01-01
Abstract
This study concerns the biochemical and physicochemical behaviour of liposomes as enzymatic bioreactors. The aim is to study a quantitative treatment of transport/kinetic phenomena in these bioreactors. The experimental system studied is the enzyme ascorbate oxidase (AAO) entrapped into dipalmitoyl-phosphatidylcholine unilamellar vesicles. The vesicles were prepared by two different methods, namely by controlled detergent dialysis technique or by reverse phase technique. A kinetic model which considers the presence of AAO both on the surface and in the aqueous core of the liposomes has been developed.File in questo prodotto:
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