The bio-hydratation of acrylonitrile, propionitrile and benzonitrile catalysed by the NHase activity contained in resting cells of Microbacterium imperiale CBS 498-74 was operated at 5degreesC, 10degreesC, and 20degreesC in laboratory scale batch or membrane bioreactors. The bioreactions were conducted in the presence of buffered media (50 mM Na2HPO4/NaH2PO4, pH 7.0) and in the presence of distilled water or tap-water, to simulate a possible end-pipe biotreatment process. Finally, the integral bioreactor performances were studied varying cell loading, from 0.1 to 16 mg(DCW)/reactor in order to realize near 100% bioconversion of acrylonitrile, propionitrile and benzonitrile without consistent loss of NHase activity
Nitrile conversion by M. imperiale CBS 498-74 resting cells in batch and UF-membrane bioreactors
CANTARELLA, Maria;GALLIFUOCO, ALBERTO;
2004-01-01
Abstract
The bio-hydratation of acrylonitrile, propionitrile and benzonitrile catalysed by the NHase activity contained in resting cells of Microbacterium imperiale CBS 498-74 was operated at 5degreesC, 10degreesC, and 20degreesC in laboratory scale batch or membrane bioreactors. The bioreactions were conducted in the presence of buffered media (50 mM Na2HPO4/NaH2PO4, pH 7.0) and in the presence of distilled water or tap-water, to simulate a possible end-pipe biotreatment process. Finally, the integral bioreactor performances were studied varying cell loading, from 0.1 to 16 mg(DCW)/reactor in order to realize near 100% bioconversion of acrylonitrile, propionitrile and benzonitrile without consistent loss of NHase activityPubblicazioni consigliate
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