In vivo biodegradation of a new haemostatic biomaterial (Matemo). *Continenza M.A., **Coppa P., *Lorenzo C., **Pajewski L.A., °D'Ascoli R., **Vegliò F. *Dip. Scienze della Salute, **Dip. Chimica, Ing. Chimica e Materiali - Università degli Studi dell'Aquila, ° Baxter Manifacturing S.p.A, Rieti (I) Introduction: Many local haemostatic agents were commercially proposed by the official pharmacopoeia to stop the bleeding during surgical interventions. In spite of this, often, the surgeon can’t easily select the best material for his specific request. More in details in fact, at the end of the intervention, a lot of the haemostatic biomaterial had to be let by the surgeon in the residual surgical cavity, to absorbe the blood, serum and other biological fluids spreading from the traumatized tissues. In this paper a comparison study was performed about the in vivo biodegradation process of two different haemostatic biomaterials: a frequently used regenerated cellulose gauze (Tabotamp), and the newly synthesized haemostatic sponge (Matemo). Materials and methods: For this experimental protocol the dorsal skin of each anesthetized animal was shaved, incised and two pouches were performed inside the subcutaneous tissue of the back, to introduce a specimen (1 cm2 of size) of Tabotamp on the left side, and one of the new biomaterial (Matemo) on the right side. The same treatment was repeated in 6 animals, and 3 other rats were used as controls, treated only with the selected anesthetic cocktail without the operation. The 6 treated animals were then divided in two groups: the first group (three animals) was killed 25 days after the operation; the second group (the other three animals) was sacrificed 45 days after the implant. All the animals were daily controlled and no accident happens during all the post-operation time. All the animals live in a good state till the two fixed dead lines, and at the end of each one, they were sacrificed by an overdose of the anesthetic drug. After death the dorsal skin of each animal was inspected in the two sides of implant and the samples from the skin and the subcutaneous tissues was taken in both sides of the back, to submit them to the histological studies. A specimen from the liver and from the kidney was also taken-off for histological controls on these organs. After formaldehyde fixation and paraffin inclusions, the slices were processed by Ematoxilin-Eosin and Azan methods, before the optical microscopy studies. Results: This protocol confirms the very interesting results obtained using the newly synthesized haemostatic biomaterial Matemo. In other preceding experiments, Matemo sponges revealed a significantly decrease of the bleeding time and its blood absorption rate was very fast, revealing the best haemostatic performances between four selected haemostatic agents in stopping the blood flow and maintaining the operation field very clean. Obviously, the faster coagulation determined the least blood loss, revealing in this way the better function in order to animal survival and to surgeon requests. The histological studies performed by this experiment show very well another aspect very important for surgical applications of this new biomaterial: the biodegradation. The inflammation developed around and the foreign body reaction toward the implant showed in this study, asses in vivo a very fast biodegradation process, in contrast with the pseudo-integration response developed around the Tabotamp in the left side of the same animal.

In vivo biodegradation of a new haemostatic biomaterial (MATEMO)

CONTINENZA, Maria Adelaide;VEGLIO', FRANCESCO
2009-01-01

Abstract

In vivo biodegradation of a new haemostatic biomaterial (Matemo). *Continenza M.A., **Coppa P., *Lorenzo C., **Pajewski L.A., °D'Ascoli R., **Vegliò F. *Dip. Scienze della Salute, **Dip. Chimica, Ing. Chimica e Materiali - Università degli Studi dell'Aquila, ° Baxter Manifacturing S.p.A, Rieti (I) Introduction: Many local haemostatic agents were commercially proposed by the official pharmacopoeia to stop the bleeding during surgical interventions. In spite of this, often, the surgeon can’t easily select the best material for his specific request. More in details in fact, at the end of the intervention, a lot of the haemostatic biomaterial had to be let by the surgeon in the residual surgical cavity, to absorbe the blood, serum and other biological fluids spreading from the traumatized tissues. In this paper a comparison study was performed about the in vivo biodegradation process of two different haemostatic biomaterials: a frequently used regenerated cellulose gauze (Tabotamp), and the newly synthesized haemostatic sponge (Matemo). Materials and methods: For this experimental protocol the dorsal skin of each anesthetized animal was shaved, incised and two pouches were performed inside the subcutaneous tissue of the back, to introduce a specimen (1 cm2 of size) of Tabotamp on the left side, and one of the new biomaterial (Matemo) on the right side. The same treatment was repeated in 6 animals, and 3 other rats were used as controls, treated only with the selected anesthetic cocktail without the operation. The 6 treated animals were then divided in two groups: the first group (three animals) was killed 25 days after the operation; the second group (the other three animals) was sacrificed 45 days after the implant. All the animals were daily controlled and no accident happens during all the post-operation time. All the animals live in a good state till the two fixed dead lines, and at the end of each one, they were sacrificed by an overdose of the anesthetic drug. After death the dorsal skin of each animal was inspected in the two sides of implant and the samples from the skin and the subcutaneous tissues was taken in both sides of the back, to submit them to the histological studies. A specimen from the liver and from the kidney was also taken-off for histological controls on these organs. After formaldehyde fixation and paraffin inclusions, the slices were processed by Ematoxilin-Eosin and Azan methods, before the optical microscopy studies. Results: This protocol confirms the very interesting results obtained using the newly synthesized haemostatic biomaterial Matemo. In other preceding experiments, Matemo sponges revealed a significantly decrease of the bleeding time and its blood absorption rate was very fast, revealing the best haemostatic performances between four selected haemostatic agents in stopping the blood flow and maintaining the operation field very clean. Obviously, the faster coagulation determined the least blood loss, revealing in this way the better function in order to animal survival and to surgeon requests. The histological studies performed by this experiment show very well another aspect very important for surgical applications of this new biomaterial: the biodegradation. The inflammation developed around and the foreign body reaction toward the implant showed in this study, asses in vivo a very fast biodegradation process, in contrast with the pseudo-integration response developed around the Tabotamp in the left side of the same animal.
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/11697/30541
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