BACKGROUND The specific Nasal Provocation Test (sNPT) is a third level diagnostic tool. SNPT has been evaluated measuring nasal resistance and nasal symptoms, reaching a 50% sensitivity. This study focused on the evaluation of three allergic response markers, triggered by nasal challenge (nasal tryptase, specific IgE, Eosinophil Cationic Protein-ECP) at the aim of increasing sNPT sensitivity. SUBJECTS AND METHOD Twenty patients suffering from allergic rhinitis and 16 age-matched not allergic subjects were enrolled in the study. The markers were evaluated by a new method, based on in situ incubation, before and after sNPT. RESULTS Tryptase levels increased in 65% of the patients, were unchanged in 20% , and slightly decreased in 15%. The increase recorded was significant in mite-allergic patients (p=0.005), but not significant (p>0.05) in pollen-allergic patients. ECP values increased in 65% of the patients, were unchanged in 10%, and highly decreased in 25%. ECP increase was not significant (p>0.05). Specific IgE levels increased in 35% of the patients, were unchanged in 55%, and decreased in 10%. The IgE increase was significant in pollen- allergic patients (p<0.05), while it was not significant in mite-allergic patients (p>0.05). The markers were not detected in the control group. CONCLUSIONS The dosage of the markers provided an additional 35% sensitivity, which together with the basal 50% reached an over-all sensitivity of 85% of the sNPT.

“Do Tryptase, ECP and specific IgE measurement by nasal incubation increase the specific nasal provocation test sensitivity?”

LAURIELLO, MARIA;
2004-01-01

Abstract

BACKGROUND The specific Nasal Provocation Test (sNPT) is a third level diagnostic tool. SNPT has been evaluated measuring nasal resistance and nasal symptoms, reaching a 50% sensitivity. This study focused on the evaluation of three allergic response markers, triggered by nasal challenge (nasal tryptase, specific IgE, Eosinophil Cationic Protein-ECP) at the aim of increasing sNPT sensitivity. SUBJECTS AND METHOD Twenty patients suffering from allergic rhinitis and 16 age-matched not allergic subjects were enrolled in the study. The markers were evaluated by a new method, based on in situ incubation, before and after sNPT. RESULTS Tryptase levels increased in 65% of the patients, were unchanged in 20% , and slightly decreased in 15%. The increase recorded was significant in mite-allergic patients (p=0.005), but not significant (p>0.05) in pollen-allergic patients. ECP values increased in 65% of the patients, were unchanged in 10%, and highly decreased in 25%. ECP increase was not significant (p>0.05). Specific IgE levels increased in 35% of the patients, were unchanged in 55%, and decreased in 10%. The IgE increase was significant in pollen- allergic patients (p<0.05), while it was not significant in mite-allergic patients (p>0.05). The markers were not detected in the control group. CONCLUSIONS The dosage of the markers provided an additional 35% sensitivity, which together with the basal 50% reached an over-all sensitivity of 85% of the sNPT.
File in questo prodotto:
Non ci sono file associati a questo prodotto.
Pubblicazioni consigliate

I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.

Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/11697/3096
Citazioni
  • ???jsp.display-item.citation.pmc??? ND
  • Scopus 15
  • ???jsp.display-item.citation.isi??? 15
social impact