We examined p53 gene that has been detected in many gastrointestinal tract neoplasms, in lO oral cancer patients. The study consisted of a primer-mediated enzymatic amplification (PCR) of two fragments (lll-319pb) included in the exon5-intron5-exon6 region, and of a successive digestion by two restriction enzymes, HhaI and Hae III. No p53 alteration was evident in any of the samples We also examined k-ras point mutations in codonl2. We agree with other authors who point out there is no evidence far alterations in the araI cavity, but these are often found in malignancies of the lower digestive tract. The absence of p53 gene modifications in oral squamous carcinomas suggests that other codon mutation or other genetic events are implicated in etiopathogenesis
P53 and H-RAS genomic analysis in oral squamous cell carcinoma
CUTILLI, Tommaso;
1991-01-01
Abstract
We examined p53 gene that has been detected in many gastrointestinal tract neoplasms, in lO oral cancer patients. The study consisted of a primer-mediated enzymatic amplification (PCR) of two fragments (lll-319pb) included in the exon5-intron5-exon6 region, and of a successive digestion by two restriction enzymes, HhaI and Hae III. No p53 alteration was evident in any of the samples We also examined k-ras point mutations in codonl2. We agree with other authors who point out there is no evidence far alterations in the araI cavity, but these are often found in malignancies of the lower digestive tract. The absence of p53 gene modifications in oral squamous carcinomas suggests that other codon mutation or other genetic events are implicated in etiopathogenesisPubblicazioni consigliate
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