PO-0999 Changes in B cell antigen expression in the elderly Ginaldi L, De Martinis M, D’Ostilio A, Marini L, Loreto MF, Profeta VF,* Quaglino D Department of Internal Medicine and Public Health, University of L’Aquila, *Ser. T ASL Teramo, Italy The involvement of the B cell compartment during senescence plays an important role in the development of the immune dysfunction (e.g. hypergammaglobulinemia, autoantibody production and impaired responses to immunisations). In order to identify specific antigen expression changes on B lymphocytes which may contribute to the immune deficiency in the elderly, we investigated, by triple staining flow cytometry, the level of expression of some constitutive surface markers (HLA-DR, CD19, CD20) and of a series of adhesion molecules (CD49b, CD49d, CD50, CD62L) on B lymphocytes from 23 healthy elderly individuals (82-100 years old, mean age 92) compared to 13 healthy young donors (20-50 years old, mean age 31). All aged donors fullfilled the admission criteria for gerontological studies proposed by the Senicor Protocol. Both percentage and absolute number of B lymphocytes (CD19+ and CD20+ cells) were significantly decreased in the elderly compared to young donors. The absolute number of B cells expressing the adhesion molecules CD49b and CD49d was lower in elderly individuals, as well as the percentage value of B cells expressing the adhesion molecules CD50 and CD62L. The percentage and absolute number of B cells coexpressing the CD5 antigen was decreased in elderly subjects. The CD20 expression antigen was increased on B lymphocytes coexpressing the CD49b and CD49d in elderly donors compared to young subjects. Also the CD5 density on B cells from old donors was slightly increased compared to controls. No differences were detected in the expression of CD19, CD50, CD49b, CD49d, CD62L and HLA-DR molecules on B lymphocytes. Quantitative flow cytometry may be of value in the elderly both for clinical and biological studies. The study of antigen density changes on B cells in the elderly may allow a better understanding of the humoral immune defects observed in these subjects and provide insights into the functional defects of the B cell compartment characterising immunosenescence.

Changes in B cell antigen expressions in the elderly

GINALDI, Lia;DE MARTINIS, MASSIMO MARIA MARCELLO;
1999-01-01

Abstract

PO-0999 Changes in B cell antigen expression in the elderly Ginaldi L, De Martinis M, D’Ostilio A, Marini L, Loreto MF, Profeta VF,* Quaglino D Department of Internal Medicine and Public Health, University of L’Aquila, *Ser. T ASL Teramo, Italy The involvement of the B cell compartment during senescence plays an important role in the development of the immune dysfunction (e.g. hypergammaglobulinemia, autoantibody production and impaired responses to immunisations). In order to identify specific antigen expression changes on B lymphocytes which may contribute to the immune deficiency in the elderly, we investigated, by triple staining flow cytometry, the level of expression of some constitutive surface markers (HLA-DR, CD19, CD20) and of a series of adhesion molecules (CD49b, CD49d, CD50, CD62L) on B lymphocytes from 23 healthy elderly individuals (82-100 years old, mean age 92) compared to 13 healthy young donors (20-50 years old, mean age 31). All aged donors fullfilled the admission criteria for gerontological studies proposed by the Senicor Protocol. Both percentage and absolute number of B lymphocytes (CD19+ and CD20+ cells) were significantly decreased in the elderly compared to young donors. The absolute number of B cells expressing the adhesion molecules CD49b and CD49d was lower in elderly individuals, as well as the percentage value of B cells expressing the adhesion molecules CD50 and CD62L. The percentage and absolute number of B cells coexpressing the CD5 antigen was decreased in elderly subjects. The CD20 expression antigen was increased on B lymphocytes coexpressing the CD49b and CD49d in elderly donors compared to young subjects. Also the CD5 density on B cells from old donors was slightly increased compared to controls. No differences were detected in the expression of CD19, CD50, CD49b, CD49d, CD62L and HLA-DR molecules on B lymphocytes. Quantitative flow cytometry may be of value in the elderly both for clinical and biological studies. The study of antigen density changes on B cells in the elderly may allow a better understanding of the humoral immune defects observed in these subjects and provide insights into the functional defects of the B cell compartment characterising immunosenescence.
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/11697/36877
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