The bioesterification of ethyl alcohol with palmitic acid catalyzed by lipase from Candida rugosa was studied. This key model reaction of biodiesel production was assayed in two different organic solvents, n-hexane and iso-octane. The activity was studied with respect to the effect of enzyme loading, and linearity conditions were determined. Substrates concentrations were varied both at stoichiometric ratio (1:1) and keeping alternatively one substrate fixed at 100 mM and varying the other one. The presence of an optimal condition (50 mM, stoichiometric ratio) was detected. The system behaves differently depending on the organic solvent, and ethanol demonstrated to have a rate depressing effect at excess concentration. These findings confirm that the kinetic pattern is complex. Finally, the effect of temperature was investigated and the activation energies in the two different organic solvent media were determined, resulting 7268 and 8763 cal/mole respectively with iso-octane and n-hexane.

A study of biochemical reaction engineering of biodisel production

M. CANTARELLA;GALLIFUOCO, ALBERTO;
2008-01-01

Abstract

The bioesterification of ethyl alcohol with palmitic acid catalyzed by lipase from Candida rugosa was studied. This key model reaction of biodiesel production was assayed in two different organic solvents, n-hexane and iso-octane. The activity was studied with respect to the effect of enzyme loading, and linearity conditions were determined. Substrates concentrations were varied both at stoichiometric ratio (1:1) and keeping alternatively one substrate fixed at 100 mM and varying the other one. The presence of an optimal condition (50 mM, stoichiometric ratio) was detected. The system behaves differently depending on the organic solvent, and ethanol demonstrated to have a rate depressing effect at excess concentration. These findings confirm that the kinetic pattern is complex. Finally, the effect of temperature was investigated and the activation energies in the two different organic solvent media were determined, resulting 7268 and 8763 cal/mole respectively with iso-octane and n-hexane.
9788895608020
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/11697/40479
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