H2O2 derived from amine-oxidase mediated oxidation of polyamines is involved in important processes, such as plant growth and development as well as biotic and abiotic stress responses. Here, our attention has been focused on the analysis of the expression of various copper-containing Amine Oxidase (AtCuAOs) and precisely AtAO1 (At4g14940), AtCuAO1 (At1g62810), AtCuAO2 (At1g31710), AtCuAO7 (At3g43670) and AtCuAO8 (At1g31690),by both mRNA level investigation and tissuespecific examination of promoter activities. AtCuAOs-promoter::GFPGUS activity analysis in different developmental stages revealed that the GUS staining and the GFP signal were detected in different tissues and organs, in particular in the root protoxylem and metaxylem tissue, cortex and epidermis, as well as in the leaf, especially stomata, hydathodes and vascular tissues, suggesting that CuAOs may play a role in differentiation of xylem tissues as well as in plant water balance. Furthermore, expression of different members of AtCuAO family is induced by hormones such as methyl-jasmonate, abscisic acid and salicylic acid suggesting a role in development and differentiation influenced by biotic and abiotic stress.

Members of AtCuAO gene family exhibit different tissue- and organ-specific pattern during seedling develiopment and distinct responses to hormone and stress treatments.

RODRIGUES POUSADA, RENATO ALBERTO;
2014-01-01

Abstract

H2O2 derived from amine-oxidase mediated oxidation of polyamines is involved in important processes, such as plant growth and development as well as biotic and abiotic stress responses. Here, our attention has been focused on the analysis of the expression of various copper-containing Amine Oxidase (AtCuAOs) and precisely AtAO1 (At4g14940), AtCuAO1 (At1g62810), AtCuAO2 (At1g31710), AtCuAO7 (At3g43670) and AtCuAO8 (At1g31690),by both mRNA level investigation and tissuespecific examination of promoter activities. AtCuAOs-promoter::GFPGUS activity analysis in different developmental stages revealed that the GUS staining and the GFP signal were detected in different tissues and organs, in particular in the root protoxylem and metaxylem tissue, cortex and epidermis, as well as in the leaf, especially stomata, hydathodes and vascular tissues, suggesting that CuAOs may play a role in differentiation of xylem tissues as well as in plant water balance. Furthermore, expression of different members of AtCuAO family is induced by hormones such as methyl-jasmonate, abscisic acid and salicylic acid suggesting a role in development and differentiation influenced by biotic and abiotic stress.
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/11697/41400
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