Ulcerative colitis (UC) is associated with low intracolonic pH and unbalance d transmucosal ionic exchange s. Along the gastrointe stinal tract carbonic anhydrase isoenzyme I (CA-I) is speci® cally expressed in colon epithe lium and is involve d in mucosal control of ion, ¯ uid, and acid± base balance . Since alte red CA-I expre ssion may play some role in UC, CA-I was measured at the mRNA and prote in level and carbonic anhydrase (CA) enzyme activity was determined in colon biopsie s of 14 UC patients (6 remission, 4 mild, 4 moderate UC) and of 12 healthy subje cts. Patients with mild or moderate UC showed a signi® cant reduction of CA-I mRNA and prote in and of total CA activity in the in¯ amed mucosa compared to controls. Patients with UC in remission showed a patte rn of CA-I expre ssion and CA activity similar to controls. This is the ® rst report showing a reduction in the expression of CA-I in active UC.

Carbonic andhydrase I reduction in colonic mucosa of patients with active ulcerative colitis

LATELLA, GIOVANNI;FRIERI, Giuseppe;
1998

Abstract

Ulcerative colitis (UC) is associated with low intracolonic pH and unbalance d transmucosal ionic exchange s. Along the gastrointe stinal tract carbonic anhydrase isoenzyme I (CA-I) is speci® cally expressed in colon epithe lium and is involve d in mucosal control of ion, ¯ uid, and acid± base balance . Since alte red CA-I expre ssion may play some role in UC, CA-I was measured at the mRNA and prote in level and carbonic anhydrase (CA) enzyme activity was determined in colon biopsie s of 14 UC patients (6 remission, 4 mild, 4 moderate UC) and of 12 healthy subje cts. Patients with mild or moderate UC showed a signi® cant reduction of CA-I mRNA and prote in and of total CA activity in the in¯ amed mucosa compared to controls. Patients with UC in remission showed a patte rn of CA-I expre ssion and CA activity similar to controls. This is the ® rst report showing a reduction in the expression of CA-I in active UC.
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Utilizza questo identificativo per citare o creare un link a questo documento: http://hdl.handle.net/11697/8193
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