PXD101 potentiates hormonal therapy and prevents the onset of castration-resistant phenotype modulating androgen receptor, HSP90, and CRM1 in preclinical models of prostate cancer.

"Aberrant activation or ‘reactivation’ of androgen receptor (AR) during androgen ablation. therapy shows a potential cause for the development of castration-resistant prostate cancer.. This study tested the hypothesis that PXD101, a potent pan histone deacetylase (HDAC). inhibitor, may prevent onset of castration-resistant phenotype and potentiate hormonal. therapy. A panel of human prostate cancer cells with graded castration-resistant phenotype. and in vivo models were used to verify this hypothesis. In this report, we demonstrated that. hormonal manipulation favors the onset of castration-resistant phenotype increasing HDAC. expression and activity as well as modulating expression and activity of AR, EGFR, HER2, and. Akt. Consistent with these observations, the functional knockdown of HDACs by PXD101. prevented the onset of castration-resistant phenotype with a significant downregulation of. AR, EGFR, HER2, and Akt expression\/activity. The dysregulation of functional cooperation. between HDAC6 with hsp90, on the one hand, and between GSK-3b with CRM1, on the. other hand, may explain the biological effects of PXD101. In this regard, the HDAC6 silencing. or the functional knockdown of hsp90 by 17AAG resulted in the selective downregulation of. AR, EGFR, HER2, and Akt expression\/activity, while the decreased phosphorylation of GSK-3b. mediated by PXD101 increased the nuclear expression of CRM1, which in turn modified the. AR and survivin recycling with increased caspase 3 activity. HDAC inhibitors retain the ability. to prevent the onset of castration-resistant phenotype and, therefore, merit clinical"