Cell fractionation is a method used to study different cellular events like protein translocation and sequestration by disrupting cells and fractionating their contents, thus allowing an enrichment of the protein of interest. Using different concentrations of sucrose or detergent buffer formulations in combination with centrifugations, the cell fractions are separated based on their density and size.

Biochemical Methods to Analyze the Subcellular Localization of NF-κB Proteins Using Cell Fractionation

Vecchiotti D.;Verzella D.;Capece D.;Di Francesco B.;Cornice J.;Alesse E.;Zazzeroni F.
2021

Abstract

Cell fractionation is a method used to study different cellular events like protein translocation and sequestration by disrupting cells and fractionating their contents, thus allowing an enrichment of the protein of interest. Using different concentrations of sucrose or detergent buffer formulations in combination with centrifugations, the cell fractions are separated based on their density and size.
978-1-0716-1668-0
978-1-0716-1669-7
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Utilizza questo identificativo per citare o creare un link a questo documento: http://hdl.handle.net/11697/180294
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