This chapter describes the isolation, culture, and staining of osteoclasts. The key advantages of this assay are that it allows direct measurement of osteoclast number, bone resorption, as well as yielding good quantities of osteoclasts at defined stages of formation for molecular analysis. An additional focus of this chapter will be the generation of osteoclasts from less conventional animal species and cell lines.

Isolation and generation of osteoclasts

Nadia Rucci
Writing – Original Draft Preparation
;
Anna Teti
Writing – Review & Editing
2019-01-01

Abstract

This chapter describes the isolation, culture, and staining of osteoclasts. The key advantages of this assay are that it allows direct measurement of osteoclast number, bone resorption, as well as yielding good quantities of osteoclasts at defined stages of formation for molecular analysis. An additional focus of this chapter will be the generation of osteoclasts from less conventional animal species and cell lines.
2019
978-1-4939-8996-6
978-1-4939-8997-3
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/11697/225099
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