Background - The AA carried out an immunohistochemical and genetic research on p53 tumor suppressor gene and H-ras oncogene in maxillofacial tumors to evaluate the p53 and H-ras status, response to induction chemotherapy, and global survival, keeping up a study performed from many years. In this paper they point out the statistical correspondence between the results of the immunohistochemical and bio-molecular analysis. Methods - 25 consecutive cases of squamous cell carcinomas with metastatic nodes were selected on the basis of homogeneous grading (G2). The patients underwent induction chemotherapy (CDDP/5FU), restaging, surgery, and adjuvant radiotherapy. Monoclonal AB, and Dna extration and PCR amplification were utilized, respectively, for immunohistochemical and genetic analysis of p53 and H-ras. Differences between proportions of contingency tables were assessed by the Fisher Exact Test; correlation analysis was done using Pearson Correlation Coefficient. A p value below 0.05 was considered statistically significant. Procedures were run by the package SAS/STAT. Results and Conclusions - The data show a strong association then between H-RAS over-expression and H-RAS genetic mutations (pFisher =0.000064) that between P53 over-expression and P53 genetic mutations (pFisher =0.000203). A significant inverse correlation was found between P53 over-expression and chemotherapy response (r= -0.914, p= 0.0001). There is a relation between the percentage of H-ras and P53 expression and genetic mutation. The 45% of linked AB in the histological specimen seems to be a significant value. Percentages greater than 45% of expressed oncogene show an high probability of genetic mutation. This condition compromises the genic product and the cellular functions related. These data show that immunohistochemical study should be inserted in the staging of tumor mostly in the advanced lesions which need multimodality of treatment. Oncogenetic pattern supplies important elements for diagnostic and prognostic evaluation, besides the classical factors of screening, and it may work together in better therapeutic planning.

Relation between p53 and H-ras overexpression and mutation in the maxillofacial tumors

CUTILLI, Tommaso;ALTOBELLI, EMMA;
2001

Abstract

Background - The AA carried out an immunohistochemical and genetic research on p53 tumor suppressor gene and H-ras oncogene in maxillofacial tumors to evaluate the p53 and H-ras status, response to induction chemotherapy, and global survival, keeping up a study performed from many years. In this paper they point out the statistical correspondence between the results of the immunohistochemical and bio-molecular analysis. Methods - 25 consecutive cases of squamous cell carcinomas with metastatic nodes were selected on the basis of homogeneous grading (G2). The patients underwent induction chemotherapy (CDDP/5FU), restaging, surgery, and adjuvant radiotherapy. Monoclonal AB, and Dna extration and PCR amplification were utilized, respectively, for immunohistochemical and genetic analysis of p53 and H-ras. Differences between proportions of contingency tables were assessed by the Fisher Exact Test; correlation analysis was done using Pearson Correlation Coefficient. A p value below 0.05 was considered statistically significant. Procedures were run by the package SAS/STAT. Results and Conclusions - The data show a strong association then between H-RAS over-expression and H-RAS genetic mutations (pFisher =0.000064) that between P53 over-expression and P53 genetic mutations (pFisher =0.000203). A significant inverse correlation was found between P53 over-expression and chemotherapy response (r= -0.914, p= 0.0001). There is a relation between the percentage of H-ras and P53 expression and genetic mutation. The 45% of linked AB in the histological specimen seems to be a significant value. Percentages greater than 45% of expressed oncogene show an high probability of genetic mutation. This condition compromises the genic product and the cellular functions related. These data show that immunohistochemical study should be inserted in the staging of tumor mostly in the advanced lesions which need multimodality of treatment. Oncogenetic pattern supplies important elements for diagnostic and prognostic evaluation, besides the classical factors of screening, and it may work together in better therapeutic planning.
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Utilizza questo identificativo per citare o creare un link a questo documento: http://hdl.handle.net/11697/7089
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