Artemisia umbelliformis subsp. eriantha is a protected species, whose essential oil is used in liqueur industry. Volatile profiles of fresh leaves and flowers from wild plants in comparison with regenerated in vitro plants introduced in experimental fields within an Italian national park were evaluated by headspace-solid phase microextraction gas chromatography mass spectrometry (HS-SPME-GC–MS). The chromatographic profiles appear to be qualitatively similar. The content of thujones, the characteristic metabolites of this species, is comparable with that obtained by analysis of essential oils. Principal component analysis of the HS-SPME-GC–MS data supports the possibility of differentiating scent blends of genetically identical plants, and even flowers and leaves from the same individual. HS-SPME-GC–MS is shown to be a very efficient method to analyse and to describe the pattern of components of A. umbelliformis subsp. eriantha cultivars’ scents. It represents a rapid screening method highlyrecommendedforthestudyofprotectedspecies,becauseitisnon-destructiveandit only requires small amounts of fresh material.

Volatiles fingerprints of Artemisia umbelliformis subs. eriantha by headspace-solid phase microextraction GC-MS

REALE, SAMANTHA;PACE, Loretta Giuseppina;D'ARCHIVIO, ANGELO ANTONIO;DE ANGELIS, Francesco;MARCOZZI, Giordana
2014-01-01

Abstract

Artemisia umbelliformis subsp. eriantha is a protected species, whose essential oil is used in liqueur industry. Volatile profiles of fresh leaves and flowers from wild plants in comparison with regenerated in vitro plants introduced in experimental fields within an Italian national park were evaluated by headspace-solid phase microextraction gas chromatography mass spectrometry (HS-SPME-GC–MS). The chromatographic profiles appear to be qualitatively similar. The content of thujones, the characteristic metabolites of this species, is comparable with that obtained by analysis of essential oils. Principal component analysis of the HS-SPME-GC–MS data supports the possibility of differentiating scent blends of genetically identical plants, and even flowers and leaves from the same individual. HS-SPME-GC–MS is shown to be a very efficient method to analyse and to describe the pattern of components of A. umbelliformis subsp. eriantha cultivars’ scents. It represents a rapid screening method highlyrecommendedforthestudyofprotectedspecies,becauseitisnon-destructiveandit only requires small amounts of fresh material.
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/11697/1153
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